EDi013-B

RCi193

iPSC line

The cell line has been recalled.

General#

Cell Line

hPSCreg Name EDi013-B
Alternative name(s)
RCi193
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University of Edinburgh (ED)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg EDi013-B
BioSamples SAMEA4459367
Cellosaurus CVCL_LE66
Wikidata Q54831994

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 60-64

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history From multiple affected family multiple affected by bipolar and major depressive disorder

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA4459366

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 60-64

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-1
No
TRA 1-60
Yes
SSEA-4
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
The probes near the telomere on the q arm of chromosome 19 indicate an increased dosage. For all other chromosomes a normal dosage pattern was observed for both the short and the long-arm probes
Passage number: 18
Karyotyping method: BACs-on-Beads

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
EDi013-B
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768