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Due to the ongoing COVID19 crisis, EBiSC is restricting non-essential work across all central biobanking sites to try and help slow spread of the disease. During this time, to minimise staff numbers within our facilities, cell lines will not be shipped. If you have ordered lines, these orders will be shipped as soon as possible after local restrictions are lifted. EBiSC can continue to help you select appropriate iPSC lines for your research and you can still apply to access managed access datasets. If you have any queries about new or ongoing orders, please contact ECACC at CultureCollections@phe.gov.uk or contact EBiSC directly.

EDi011-C

Donor information
Gender:	Male
Disease status
Disease:	bipolar disorder Brief psychotic episode
Affected:	Yes
Family history:	From multiple affected family multiple affected by bipolar and major depressive disorder
General information
Depositor:	University of Edinburgh
Cell line name:	EDi011-C
Cell line alternative names:	RCi141
Biosamples ID:	SAMEA4459360
hPSCreg name:	EDi011-C
Related lines
From the same donor:	EDi011-A, EDi011-B

Derivation

Derivation
Primary cell type:	Fibroblast of dermis
Location of primary tissue procurement:	Zone of skin
Reprogramming method
Vector type:	Non-integrating
Vector:	Sendai virus
Gene list:	KLF4 MYC POU5F1 SOX2
Is the reprogramming vector detectable:	Unknown
Derivation
Xeno free conditions:	no
Derived under gmp:	no
Available as clinical grade:	no

Culture conditions

Culture Conditions
Surface coating:	Vitronectin
Passage method:	Enzyme-free cell dissociation / EDTA
O₂ concentration:	20%
CO₂ concentration:	5%
Culture medium:	Essential 8™

Characterization

Characterization

Analysis of Undifferentiated Cells

Marker expression:

Marker	Expressed	Immunostaining	RT-PCR	FACS	Enzymatic Assay	Expression Profiles
TRA 1-60	Yes
SSEA-1	Yes
SSEA-4	Yes
POU5F1 (OCT-4)	Yes

Microbiology / Virus Screening

HIV 1:

Negative

HIV 2:

Not done

Hepatitis B:

Negative

Hepatitis C:

Negative

Mycoplasma:

-

Genotyping

Genotyping
Karyotyping
Passage number:	26
Cell line karyotype:	No autosomal or sex chromosome aneuploidies detected
Karyotyping method:	Karyolite BoBs
Genotyping
Genome-wide analysis:	Whole genome sequencing https://ega-archive.org/studies/EGAS00001002755

At European Collection of Authenticated Cell Cultures (ECACC)