EDi011-C
Donor information | |
|---|---|
| Gender: | Male |
Disease status | |
| Disease: |
bipolar disorder
Brief psychotic episode |
| Affected: | Yes |
| Family history: | From multiple affected family multiple affected by bipolar and major depressive disorder |
General information | |
| Depositor: | University of Edinburgh |
| Cell line name: | EDi011-C |
| Cell line alternative names: | RCi141 |
| Biosamples ID: | SAMEA4459360 |
| hPSCreg name: | EDi011-C |
Related lines | |
| From the same donor: | EDi011-A, EDi011-B |
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Derivation
Derivation
Primary cell type: Fibroblast of dermis Location of primary tissue procurement: Zone of skin Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Surface coating: Vitronectin Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 20% CO2 concentration: 5% Culture medium: Essential 8™ -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles SSEA-4 Yes TRA 1-60 Yes SSEA-1 Yes POU5F1 (OCT-4) Yes Microbiology / Virus Screening
HIV 1: Negative HIV 2: Not done Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: - -
Genotyping
Genotyping
Karyotyping
Passage number: 26 Cell line karyotype: No autosomal or sex chromosome aneuploidies detected Karyotyping method: Karyolite BoBs Genotyping
Genome-wide analysis: Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
At European Collection of Authenticated Cell Cultures (ECACC)
Restocking
Cell Line Information Pack
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