EDi010-B

RCi138

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name EDi010-B
Alternative name(s)
RCi138
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University of Edinburgh (ED)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg EDi010-B
BioSamples SAMEA4459356
Cellosaurus CVCL_LE58
ECACC 66540448
Wikidata Q54831986

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
bipolar disorder
Bipolar Disorder, type I
The donor is affected.
Synonyms
  • Psychoses, Manic-Depressive
  • Bipolar affective disorder, current episode depression (disorder)
  • Manic bipolar I disorder
  • Manic-depressive psychosis
  • mixed bipolar affective disorder, NOS (disorder)
  • Disorder, Bipolar
  • Manic bipolar I disorder (disorder)
  • Manias
  • Bipolar Disorders
  • Affective Bipolar Psychosis
  • Psychosis, Bipolar Affective
  • Psychosis, Manic-Depressive
  • Manic Depressive Psychosis
  • MANIC DIS
  • Bipolar Depression
  • BIPOLAR DIS
  • Manic Disorders
  • Unspecified bipolar affective disorder, NOS (disorder)
  • Bipolar affective disorder
  • Manic States
  • Unspecified bipolar affective disorder
  • State, Manic
  • Psychoses, Manic Depressive
  • MANIC DEPRESSIVE ILLNESS
  • Mania
  • bipolar disorder manic phase
  • Unspecified bipolar affective disorder, NOS
  • Psychoses, Bipolar Affective
  • Unspecified bipolar affective disorder, unspecified (disorder)
  • Unspecified bipolar affective disorder, unspecified
  • Psychosis, Manic Depressive
  • Bipolar affective disorder , current episode mixed (disorder)
  • Disorder, Manic
  • Manic-Depressive Psychoses
  • Manic Disorder
  • States, Manic
  • mixed bipolar disorder
  • [X]Bipolar affective disorder, unspecified (disorder)
  • Unspecified bipolar affective disorder (disorder)
  • Bipolar disorder, unspecified
  • Bipolar Affective Psychosis
  • Manic-depressive syndrome NOS
  • Manic Bipolar Affective disorder
  • Manic State
  • Bipolar affective disorder, manic, unspecified degree
  • Bipolar disorder (disorder)
  • mixed bipolar affective disorder (disorder)
  • Affective Psychosis, Bipolar
  • [X]Bipolar affective disorder, unspecified
  • bipolar disease
  • Bipolar affective disorder, mixed, unspecified degree
  • MDI - Manic-depressive illness
  • Manic-depressive illness
  • Bipolar disorder, NOS
  • BIPOLAR DISORDER NOS
  • mixed bipolar I disorder (disorder)
  • Depression, Bipolar
  • Depressive-manic psych.
  • Manic-Depression
  • manic-depression
  • major affective disorder
  • bipolar depression
  • bipolar disorder
  • Manic Depressive disorder
show more synonyms
Family history From multiple affected family multiple affected by bipolar and major depressive disorder

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA4459355

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SSEA-4
Yes
SSEA-1
No

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No autosomal or sex chromosome aneuploidies detected
Passage number: 16
Karyotyping method: Karyolite BoBs

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768