EDi002-A

NAS2

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name EDi002-A
Alternative name(s)
NAS2
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University of Edinburgh (ED)
Owner College of Medicine and Veterinary Medicine
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Roslin Cells (RC)
Derivation country United States

External Databases

hPSCreg EDi002-A
BioSamples SAMEA3333374
Cellosaurus CVCL_AW98
ECACC 66540059
CLO CLO_0100622
Wikidata Q54831976

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 30-34

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes
  • Daughter of AST woman (EDi001-A), who has PD with SNCA triplication.This donor did not inherit the mutation.
Family history Strong family history of Parkinson’s disease due to autosomal dominant inheritance of SNCA triplication. This volunteer did not inherit the mutation

Donor Relations

All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA3333373

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 30-34

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Laminin
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 95 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Mesoderm
Ont Id: UBERON_0000926
Ectoderm
Ont Id: UBERON_0000924

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 48
Karyotyping method: Molecular karyotyping by SNP array
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM701392

Other Genotyping (Cell Line)