CENSOi008-A

FB77R2c3, CENSOi260

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
Timepoint: Confluence
Magnification: x4
Timepoint: Confluence
Magnification: x10
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name CENSOi008-A
Alternative name(s)
FB77R2c3, CENSOi260
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Censo Biotechnologies (CENSO)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg CENSOi008-A
BioSamples SAMEA104133816
Cellosaurus CVCL_LE91
ECACC 66540603
CLO CLO_0101674
Wikidata Q54809301

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 25-29

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
myotonic dystrophy type 1
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Dystrophia myotonica
  • congenital myotonic dystrophy
  • Steinert disease
  • myotonic dystrophy of Steinert
show more synonyms
Genetic variants
DMPK (target)
DMPK expansion mutation

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

External Databases (Donor)

BioSamples SAMEA104133817

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 25-29
Collected in 2015

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA
mRNA

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
SSEA-1
No
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
MIXL1
Yes
GATA6
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
NCAM1
Yes
VIM
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
NeuroD1
Yes
HES5
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No autosomal or sex aneuploidies were detected in this sample
Passage number: 10
Karyotyping method: KaryoLite BoBs

Other Genotyping (Cell Line)