CENSOi007-A
Donor information | |
|---|---|
| Gender: | Male |
| Age: | 10-14 |
Disease status | |
| Disease: | DMD |
| Affected: | Yes |
| Carrier: | Yes |
| Disease variant | |
| Gene: | DMD |
| Chromosome location: | Xp21.2-p21.1 |
| Description: | A deletion of exon 52 of the dystrophin gene |
| Disease associated phenotypes: | Steroid therapy; Prednisolone 22.5mg (10 days on/10 days off); decline in motor abilities 2 years after starting steroid therapy; can still walk independently at time of sample collection. |
General information | |
| Depositor: | Censo Biotechnologies |
| Cell line name: | CENSOi007-A |
| Cell line alternative names: | FB75R2c5, CENSOi255 |
| Biosamples ID: | SAMEA104133814 |
| hPSCreg name: | CENSOi007-A |
Timepoint: Confluence
Magnification: x4
Timepoint: Confluence
Magnification: x10
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Derivation
Derivation
Primary cell type: Fibroblast Tissue collection year: 2014 Passage number reprogrammed: 5 Reprogramming method
Vector type: None Vector Free Reprogramming
Types of factor(s) used: mRNA mRNA: POU5F1
SOX2
KLF4
MYC
NANOG
LIN28Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Medium: mTeSR Passage method: EDTA Matrix: Matrigel / Geltrex CO2 concentration: 5% O2 concentration: 21% Temperature: 37C -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles TRA 1-60 Yes SSEA-1 No POU5F1 (OCT-4) Yes SSEA-4 Yes Differentiation potency
Ectoderm: Ectoderm In vitro spontaneous differentiation
Marker Expressed HES5 Yes NeuroD1 Yes PAX6 Yes Endoderm: Endoderm In vitro spontaneous differentiation
Marker Expressed CXCR4 Yes GATA6 Yes Mesoderm: Mesoderm In vitro spontaneous differentiation
Marker Expressed NCAM1 Yes VIM Yes Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Sterility
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation -
Genotyping
Genotyping
Karyotyping
Passage number: 11 Cell line karyotype: No autosomal or sex chromosome aneuploidies were detected Karyotyping method: KaryoLite BoBs Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
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