BIONi037-A
Donor information | |
|---|---|
| Gender: | Female |
| Age: | 75-79 |
| Ethnicity: | Caucasian |
Disease status | |
| Disease: | normal |
General information | |
| Depositor: | Bioneer |
| Cell line name: | BIONi037-A |
| Cell line alternative names: | 16423 ApoE3/3 (control line) |
| Biosamples ID: | SAMEA104026330 |
| hPSCreg name: | BIONi037-A |
| Derivation country: | Denmark |
| Reference publications: | |
Related lines | |
| Subclones: | BIONi037-A-1, BIONi037-A-2, BIONi037-A-3, BIONi037-A-4 |
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Derivation
Derivation
Primary cell type: Fibroblast of dermis Location of primary tissue procurement: Zone of skin Tissue collection year: 2010 Passage number reprogrammed: 18 Reprogramming method
Vector type: Non-integrating Vector: Episomal Gene list: - KLF4
- Lin28
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: No Merthods used: pcr Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Surface coating: Matrigel/Geltrex Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 18% CO2 concentration: 5% Culture medium: Essential 8™ Passage Number banked (pre-EBiSC): 18 No. Vials banked (pre-EBiSC): 30 -
Characterization
Characterization
Analysis of Undifferentiated Cells
Morphology: Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative -
Genotyping
Genotyping
Karyotyping
Passage number: 25 Cell line karyotype: 46, XX Karyotyping method: G-Banding Image / Result Genotyping
Genome-wide analysis: Sanger Sequencing
Genome-wide analysis (donor): Sanger sequencing
Data file
At European Collection of Authenticated Cell Cultures (ECACC)
Restocking
Cell Line Information Pack
A Cell Line Information Pack (CLIP) is created to communicate cell line specific information relating to restrictions on use to a user accessing cell lines from EBiSC. A CLIP contains information about a cell line including any specific third party obligations (TPOs) relating to, for example, intellectual property (IP) or the donor consent which affect the use of the cell line.