BIONi037-A
16423 ApoE3/3 (control line)
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg Name | BIONi037-A |
| Alternative name(s) |
16423 ApoE3/3 (control line)
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
|
| Depositor | Bioneer (BION) |
| Owner | Bioneer (BION) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
| Derivation country | Denmark |
External Databases |
|
| hPSCreg | BIONi037-A |
| BioSamples | SAMEA104026330 |
| ECACC | 66540580 |
| Cellosaurus | CVCL_II80 |
| CLO | CLO_0100582 |
| Wikidata | Q54796873 |
General Information |
|
| Reference Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: allowed
Commercial: allowed
|
| Subclones | |
Donor Information#
General Donor Information |
|
| Sex | female |
| Age of donor (at collection) | 75-79 |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Unknown
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Sanger sequencing
The donor of this line carries the ApoE3/E3 allele. vcf File: |
Donor Relations |
|
| Other cell lines of this donor |
|
External Databases (Donor) |
|
| BioSamples | SAMEA104026331 |
hIPSC Derivation#
General |
|
| Source cell type |
fibroblast of dermis |
| Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
|
| Age of donor (at collection) | 75-79 |
| Collected in | 2010 |
| Passage number reprogrammed | 18 |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
Vector free reprogramming |
|
Other |
|
| Selection criteria for clones | morphology |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
Yes |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 18 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Morphology pictures
Tuj1, SMA and AFP have been analysed after spontaneous differentiation as embryoic bodies. See publication:
https://doi.org/10.1016/j.scr.2016.09.019
Method documentation
Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Sterility |
|
| Inoculation for microbiological growth | No Contaminants Detected |
| Mycoplasma | Not Detected |
| Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Sanger Sequencing
The genotype at base position described by rs429358 is T/T and by rs7412 is C/C (ApoE3/E3). |