BIONi037-A
16423 ApoE3/3 (control line)
iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.
General#
Cell Line |
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hPSCreg Name | BIONi037-A |
Alternative name(s) |
16423 ApoE3/3 (control line)
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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Depositor | Bioneer (BION) |
Owner | Bioneer (BION) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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Derivation country | Denmark |
External Databases |
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hPSCreg | BIONi037-A |
BioSamples | SAMEA104026330 |
ECACC | 66540580 |
Cellosaurus | CVCL_II80 |
CLO | CLO_0100582 |
Wikidata | Q54796873 |
General Information |
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Reference Publications | |
* Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: allowed
Commercial: allowed
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Subclones |
Donor Information#
General Donor Information |
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Sex | female |
Age of donor (at collection) | 75-79 |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
Yes
Sanger sequencing
The donor of this line carries the ApoE3/E3 allele. vcf File: |
Donor Relations |
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Other cell lines of this donor |
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External Databases (Donor) |
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BioSamples | SAMEA104026331 |
hIPSC Derivation#
General |
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Source cell type |
fibroblast of dermis |
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
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Age of donor (at collection) | 75-79 |
Collected in | 2010 |
Passage number reprogrammed | 18 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Genes | |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Vector free reprogramming |
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Other |
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Selection criteria for clones | morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
Yes |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 18 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Morphology pictures
Tuj1, SMA and AFP have been analysed after spontaneous differentiation as embryoic bodies. See publication:
https://doi.org/10.1016/j.scr.2016.09.019
Method documentation
Differentiation Potency
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Sterility |
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Inoculation for microbiological growth | No Contaminants Detected |
Mycoplasma | Not Detected |
Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Is there genome-wide genotyping or functional data available? |
Yes
Sanger Sequencing
The genotype at base position described by rs429358 is T/T and by rs7412 is C/C (ApoE3/E3). |