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Due to the ongoing COVID19 crisis, EBiSC is restricting non-essential work across all central biobanking sites to try and help slow spread of the disease. During this time, to minimise staff numbers within our facilities, cell lines will not be shipped. If you have ordered lines, these orders will be shipped as soon as possible after local restrictions are lifted. EBiSC can continue to help you select appropriate iPSC lines for your research and you can still apply to access managed access datasets. If you have any queries about new or ongoing orders, please contact ECACC at CultureCollections@phe.gov.uk or contact EBiSC directly.

BIONi037-A

Donor information
Gender:	Female
Age:	75-79
Ethnicity:	Caucasian
Disease status
Disease:	normal
General information
Depositor:	Bioneer
Cell line name:	BIONi037-A
Cell line alternative names:	16423 ApoE3/3 (control line)
Biosamples ID:	SAMEA104026330
hPSCreg name:	BIONi037-A
Derivation country:	Denmark
Reference publications:	Nimsanor N et al. Generation of induced pluripotent stem cells derived from a 77-year-old healthy woman as control for age related diseases. Stem cell research. 2016 Nov;17(3):550-552.
Related lines
Subclones:	BIONi037-A-1, BIONi037-A-2, BIONi037-A-3, BIONi037-A-4

Derivation

Derivation
Primary cell type:	Fibroblast of dermis
Location of primary tissue procurement:	Zone of skin
Tissue collection year:	2010
Passage number reprogrammed:	18
Reprogramming method
Vector type:	Non-integrating
Vector:	Episomal
Gene list:	KLF4 Lin28 MYC POU5F1 SOX2
Is the reprogramming vector detectable:	No
Merthods used:	pcr
Derivation
Xeno free conditions:	no
Derived under gmp:	no
Available as clinical grade:	no

Culture conditions

Culture Conditions
Surface coating:	Matrigel/Geltrex
Passage method:	Enzyme-free cell dissociation / EDTA
O₂ concentration:	18%
CO₂ concentration:	5%
Culture medium:	Essential 8™
Passage Number banked (pre-EBiSC):	18
No. Vials banked (pre-EBiSC):	30

Characterization

Characterization
Analysis of Undifferentiated Cells
Morphology:	07-25-2017 16423 apoe 33 .tif
Microbiology / Virus Screening
HIV 1:	Negative
HIV 2:	Negative
Hepatitis B:	Negative
Hepatitis C:	Negative
Mycoplasma:	Negative

Genotyping

Genotyping
Karyotyping
Passage number:	25
Cell line karyotype:	46, XX
Karyotyping method:	G-Banding
	Image / Result
Genotyping
Genome-wide analysis:	Sanger Sequencing
Genome-wide analysis (donor):	Sanger sequencing Data file

At European Collection of Authenticated Cell Cultures (ECACC)