BIONi037-A

16423 ApoE3/3 (control line)

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name BIONi037-A
Alternative name(s)
16423 ApoE3/3 (control line)
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Bioneer (BION)
Owner Bioneer (BION)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country Denmark

External Databases

hPSCreg BIONi037-A
BioSamples SAMEA104026330
ECACC 66540580
Cellosaurus CVCL_II80
CLO CLO_0100582
Wikidata Q54796873

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed
Subclones

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 75-79
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Sanger sequencing
The donor of this line carries the ApoE3/E3 allele.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104026331

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 75-79
Collected in 2010
Passage number reprogrammed 18

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
No
Derived under GMP?
Yes
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 18 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Tuj1, SMA and AFP have been analysed after spontaneous differentiation as embryoic bodies. See publication: https://doi.org/10.1016/j.scr.2016.09.019
Method documentation
Differentiation Potency

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: 25
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Sanger Sequencing
The genotype at base position described by rs429358 is T/T and by rs7412 is C/C (ApoE3/E3).