BIONi018-A
Donor information | |
|---|---|
| Gender: | Male |
| Age: | 20-24 |
Disease status | |
| Health Status: | normal |
General information | |
| Depositor: | Bioneer |
| Cell line name: | BIONi018-A |
| Cell line alternative names: | H050815 48-4, SAMEA4451665 |
| Biosamples ID: | SAMEA4451665 |
| hPSCreg name: | BIONi018-A |
| Derivation country: | Denmark |
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Derivation
Derivation
Primary cell type: Adipose tissue derived mesenchymal stem cell Location of primary tissue procurement: Adipose tissue Passage number reprogrammed: 3 Reprogramming method
Vector type: Non-integrating Vector: Episomal Gene list: - KLF4
- LIN28
- MYC
- POU5F1
- shP53
- SOX2
Is the reprogramming vector detectable: No Merthods used: pcr Notes on reprogramming vector detection: DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control. Vector map: File Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Medium: Essential E8 Passage method: EDTA Matrix: Matrigel / Geltrex CO2 concentration: 5% O2 concentration: 5% Temperature: 37C -
Characterization
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Genotyping
Genotyping
Karyotyping
Passage number: 11 Cell line karyotype: 46, XY, normal male karyotype Karyotyping method: G-Banding Image / Result Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
Cell Line Information Pack
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A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.