BIONi018-A

H050815 48-4, SAMEA4451665

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name BIONi018-A
Alternative name(s)
H050815 48-4, SAMEA4451665
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Bioneer (BION)
Owner Bioneer (BSC)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country Denmark

External Databases

hPSCreg BIONi018-A
BioSamples SAMEA4451665
Cellosaurus CVCL_LE16
ECACC 66540361
CLO CLO_0100564
Wikidata Q54796799

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 20-24

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA4451664

hIPSC Derivation#

General

Source cell type
adipose tissue derived mesenchymal stem cell
An adult mesenchymal stem cell derived from adipose tisssue.
Synonyms
  • human mesenchymal stem cell from adipose tissue
  • hMSC-AT
Source cell origin
adipose tissue
Portion of connective tissue composed of adipocytes enmeshed in areolar tissue.
Synonyms
  • fat
  • fatty tissue
  • fat tissue
Source cell type (free text) Pre-Adipocyte
Age of donor (at collection) 20-24
Passage number reprogrammed 3

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control.
Vector map

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium Essential E8
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 5
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-1
No
SSEA-4
Yes
SSEA-4
Yes
TRA 1-81
Yes
TDGF1
Yes
GDF3
Yes
GABRB3
Yes
DMNT3B
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: NCRO_0000112
In vitro directed differentiation
Marker Expressed
CXCR4
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
CD34
Yes
CD56
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
PAX6
Yes
NES
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY, normal male karyotype
Passage number: 11
Karyotyping method: G-Banding

Other Genotyping (Cell Line)