|Cell line name:||BIONi015-A|
|Cell line alternative names:||H280715 47-1, SAMEA4342649|
Primary cell type: Adipose tissue derived mesenchymal stem cell Location of primary tissue procurement: Adipose tissue Passage number reprogrammed: 3
Vector type: Non-integrating Vector: Episomal Gene list:
Is the reprogramming vector detectable: No Merthods used: pcr Notes on reprogramming vector detection: DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control. Vector map: File
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no
Medium: Essential E8 Passage method: EDTA Matrix: Matrigel / Geltrex CO2 concentration: 5% O2 concentration: 5% Temperature: 37C
Cell line karyotype: 46, XY, normal male karyotype Karyotyping method: G-Banding
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Cell Line Information Pack
A Cell Line Information Pack (CLIP) is created to communicate cell line specific information relating to restrictions on use to a user accessing cell lines from EBiSC. A CLIP contains information about a cell line including any specific third party obligations (TPOs) relating to, for example, intellectual property (IP) or the donor consent which affect the use of the cell line.