|Cell line name:||BIONi013-A|
|Cell line alternative names:||H240715 47-1, SAMEA4342502|
Primary cell type: Adipose tissue derived mesenchymal stem cell Location of primary tissue procurement: Adipose tissue Passage number reprogrammed: 3
Vector type: Non-integrating Vector: Episomal Gene list:
Is the reprogramming vector detectable: No Merthods used: pcr Notes on reprogramming vector detection: DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control. Vector map: File
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no
Medium: Essential E8 Passage method: EDTA Matrix: Matrigel / Geltrex CO2 concentration: 5% O2 concentration: 5% Temperature: 37C
Passage number: 12 Cell line karyotype: 46, XY normal male karyotype Karyotyping method: G-Banding Image / Result
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.
Cell Line Information Pack
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