BIONi010-C-8 Gene-edited iPSC line
Donor information | |
|---|---|
| Gender: | Male |
| Donor karyotype: | 46, XY |
Disease status | |
Donor | |
| Disease: | normal |
Disease/phenotype related genetic modifications |
|
| Disease: | Alzheimer's disease |
| Type of modification: | Isogenic |
| Gene: | TREM2 |
| Chromosome location: | 6p21.1 |
| Nucleotide sequence HGSV: | NM_018965.3:c.197C>T |
| Protein sequence HGSV: | NP_061838.1:p.Thr66Met |
| Zygosity: | Homozygous |
| Target locus modification: | Mutated |
| Description: | A homozygous T66M (rs201258663) mutation was interted into the TREM2 locus of BIONi010-C |
General information | |
| Depositor: | Bioneer |
| Cell line name: | BIONi010-C-8 |
| Cell line alternative names: | BIONi010-C Trem2 T66M, #Y5-80 |
| Biosamples ID: | SAMEA4454011 |
| hPSCreg name: | BIONi010-C-8 |
Related lines | |
| Derived from: | BIONi010-C |
| All subclones of BIONi010-C: | BIONi010-C-10, BIONi010-C-11, BIONi010-C-12, BIONi010-C-13, BIONi010-C-15, BIONi010-C-17, BIONi010-C-18, BIONi010-C-19, BIONi010-C-2, BIONi010-C-24, BIONi010-C-25, BIONi010-C-3, BIONi010-C-4, BIONi010-C-5, BIONi010-C-6, BIONi010-C-7, BIONi010-C-8, BIONi010-C-9 |
| From the same donor: | BIONi010-A, BIONi010-B |
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Derivation
Derivation
Primary cell type: - Reprogramming method
Vector type: Non-integrating Vector: Episomal Gene list: - KLF4
- Lin28
- MYC
- POU5F1
- shP53
- SOX2
Is the reprogramming vector detectable: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Surface coating: Matrigel/Geltrex Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 18% CO2 concentration: 5% Culture medium: Essential 8™ Passage Number banked (pre-EBiSC): 24 No. Vials banked (pre-EBiSC): 20 -
Characterization
Characterization
Analysis of Undifferentiated Cells
Morphology: 
Differentiation potency
Ectoderm: Ectoderm Endoderm: Endoderm Mesoderm: Mesoderm Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative Sterility
Inoculation for microbiological growth: No Contaminants Detected Mycoplasma: Not Detected Viability: Viable post-cryopreservation -
Genotyping
Genotyping
Karyotyping
Cell line karyotype: 46, XY Karyotyping method: G-Banding Image / Result Genotyping
Genome-wide analysis: Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase. -
Genetic modification
Genetic modification
Disease/phenotype related modifications
Disease: Alzheimer's disease Type of modification: Isogenic Gene: TREM2 Chromosome location: 6p21.1 Nucleotide sequence HGSV: NM_018965.3:c.197C>T Protein sequence HGSV: NP_061838.1:p.Thr66Met Zygosity: Homozygous Target locus modification: Mutated Description: A homozygous T66M (rs201258663) mutation was interted into the TREM2 locus of BIONi010-C
No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
Cell Line Information Pack
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