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BIONi010-C-6 Gene-edited iPSC line

Donor information

Gender:Male
Donor karyotype:46, XY

Disease status

Donor

Disease: normal

Disease/phenotype related genetic modifications

Disease: Alzheimer's disease
Type of modification: Isogenic
Gene: ApoE
Chromosome location: 19q13.3
Nucleotide sequence HGSV: NM_000041.4:c.388C>T
Protein sequence HGSV: NP_000032.1:p.Arg130Cys
Zygosity: Homozygous
Target locus modification: Changed
Description: BIONi010-C-6 ApoE2/E2 is an isogenic mutant of BIONi010-C (ApoE3/E4); The genotype at base position described by rs429358 has been modified in the subclone from T/C to T/T, which changes aa from Arg to Cys. Together with other base changes at rs7412 these define a ApoE2/E2 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone.

Type of modification: Isogenic
Gene: ApoE
Chromosome location: 19q13.3
Nucleotide sequence HGSV: NM_000041.3:c.526C>T
Protein sequence HGSV: NP_000032.1:p.Arg176Cys
Zygosity: Homozygous
Target locus modification: Changed
Description: BIONi010-C-6 ApoE2/E2 is an isogenic mutant of BIONi010-C (ApoE3/E4); The genotype at base position described by rs7412 has been modified in the subclone from C/C to T/T, which changes aa from Arg to Cys. Together with other base changes at rs429358 these define a ApoE2/E2 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone.

General information

Depositor:Bioneer
Cell line name:BIONi010-C-6
Cell line alternative names:BIONi010-C ApoE E2/E2
Biosamples ID:SAMEA4454009
hPSCreg name:BIONi010-C-6
Derivation country:Denmark

Related lines

Derived from: BIONi010-C
All subclones of BIONi010-C: BIONi010-C-10, BIONi010-C-11, BIONi010-C-12, BIONi010-C-13, BIONi010-C-15, BIONi010-C-17, BIONi010-C-18, BIONi010-C-19, BIONi010-C-2, BIONi010-C-24, BIONi010-C-25, BIONi010-C-3, BIONi010-C-4, BIONi010-C-5, BIONi010-C-6, BIONi010-C-7, BIONi010-C-8, BIONi010-C-9
From the same donor: BIONi010-A, BIONi010-B
Timepoint: Confluence
Magnification: x4
Timepoint: Confluence
Magnification: x10
This line is part of a set of isogenic APOE lines based on the iPS cell line BIONi010-C. The set comprises the following APOE genotypes: • BIONi010-C-6 (APOE 2/2) • BIONi010-C-2 (APOE 3/3) • BIONi010-C (APOE 3/4) • BIONi010-C-4 (APOE 4/4) • BIONi010-C-3 (APOE KO) A DNA SNP array revealed no larger chromosomal aberrations to be reported. An unsignificant duplication of 1,4Mbp was found on Chr22 in q11.23. This duplication was found in all isogenic lines generated from BIONi010-C as well as BIONi010-C. Other isogenic ApoE cell line cohorts are also available, generated from BIONi37-A, UKBi011-A and STBci006-A.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
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