BIONi010-C-48

BIONi010-C hMDR1

General

Cell Line

hPSCreg name BIONi010-C-48
Cite as:
BIONi010-C-48
Alternative name(s)
BIONi010-C hMDR1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
BIONi010-C
(BIONi010-C, K3P53)
BIONi010-C-51
(BIONi010-C TNNI3-mCherry reporter)
BIONi010-C-13
(BIONi010-C + NGN2 #I7-26)
BIONi010-C-2
(BIONi010-C ApoE E3/E3 #H8 P32)
BIONi010-C-18
(BIONi010-C TBK1 KO)
BIONi010-C-3
(BIONi010-C ApoE KO #KO30 P30)
BIONi010-C-19
(BIONi010-C IKBKE KO)
BIONi010-C-43
(BIONi010-C + aSNCA-wt AAVS1)
BIONi010-C-15
(BIONi010-C +dox inducible NGN2-GFP)
BIONi010-C-44
(BIONi010-C + aSNCA-A53T AAVS1)
BIONi010-C-64
(BIONi010-C-T2A-Nanoluciferase reporter cl. 29)
BIONi010-C-25
(BIONi010-C heterozygous TREM2 KO)
BIONi010-C-10
(HNF1AP291fsinsC +/- 54-5)
BIONi010-C-11
(HNF1AP291fsinsC -/- 66-1)
BIONi010-C-12
(HNF4ApR309C -/- 2-4)
BIONi010-C-6
(BIONi010-C ApoE E2/E2)
BIONi010-C-7
(BIONi010-C Trem2 R47H)
BIONi010-C-8
(BIONi010-C Trem2 T66M, #Y5-80)
BIONi010-C-9
(BIONi010-C CD33 KO)
BIONi010-C-49
(BIONi010-C + synapsin-m2rtTA + SNCA-wt)
BIONi010-C-17
(BIONi010-C TREM2 KO)
BIONi010-C-50
(BIONi010-C + synapsin-m2rtTA + SNCA-A53T)
BIONi010-C-56
(BIONi010-C-A713T-C25)
BIONi010-C-57
(BIONi010-C-A713T-C42)
BIONi010-C-52
(BIONi010-C with an APOE 2/2 genotype (with two functional alleles in contrast to BIONi010-C-6))
BIONi010-C-70
(BIONi010-C with an APOE 2/2 genotype with an additional, homozygous christchurch mutation)
BIONi010-C-53
(BIONi010-C with an APOE 3/3 genotype (with two functional alleles in contrast to BIONi010-C-2))
BIONi010-C-58
(BIONi010-C-A713T-C1)
BIONi010-C-71
(BIONi010-C with an APOE 3/3 genotype with an additional, homozygous christchurch mutation)
BIONi010-C-54
(BIONi010-C with an APOE 4/4 genotype (with two functional alleles in contrast to BIONi010-C-4))
BIONi010-C-55
(BIONi010-C TNNI3-mCherry/TNNI1-EGFP dual reporter cl. 74)
BIONi010-C-59
(BIONi010-C-A713T-C33)
BIONi010-C-60
(BIONi010-C-R589C-C7)
BIONi010-C-61
(BIONi010-C-R589C-C16)
BIONi010-C-62
(BIONi010-C-R589C-C5)
BIONi010-C-63
(BIONi010-C-R589C-C9)
BIONi010-C-24
(BIONi010-C Dox a-syn)
BIONi010-C-45
(BIONi010-C iCRE AAVS1 GBA1 LoxP EX5-6)
BIONi010-C-41
(BIONi010-C + iNGN2 Two-plasmid system/CRISPR-2)
BIONi010-C-65
(BiONI010-C-O16)
BIONi010-C-66
(BIONi010-C-N7)
BIONi010-C-4
(BIONi010-C ApoE E4/E4 #B44 P27)
BIONi010-C-42
(BIONi010-C + iCRE AAVS1)
BIONi010-C-5
(BIONi010-C CD33 E2del #N14 P26)
BIONi010-A
(K1P53)
BIONi010-B
(K2P53, BIONi010-B)
Last update 9th February 2024
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Provider

Generator Bioneer (BION)
Contact:
Bioneer (BION)
Owner Bioneer (BION)
Derivation country Denmark

External Databases

BioSamples SAMEA115286272

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Black or African-American

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

Ethics

Also have a look at the ethics information for the parental line BIONi010-C .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

The source cell information can be found in the parental cell line BIONi010-C.
Passage number reprogrammed 27

Reprogramming method

Vector type Non-integrating
Vector Episomal
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Accutase
Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-81
Yes
SOX2
Yes
NANOG
Yes
SSEA-3
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Morphology

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 27
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
Transgene expression
AAVS1
hMDR1 gene was inserted into the AAVS1 locus. Expression is constitutively active.
TALEN