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BIONi010-C-4 Gene-edited iPSC line

Donor information

Donor karyotype:46, XY

Disease status


Disease: normal

Disease/phenotype related genetic modifications

Disease: Alzheimer's disease
Type of modification: Isogenic
Gene: ApoE
Chromosome location: 19q13.3
Nucleotide sequence HGSV: NM_000041.4:c.388T>C
Protein sequence HGSV: NP_000032.1:p.Cys130Arg
Zygosity: Homozygous
Target locus modification: Changed
Description: BIONi010-C-4 ApoE4/E4 is an isogenic mutant of BIONi010-C (ApoE3/E4); The genotype at base position described by rs429358 has been modified in the subclone from T/C to C/C, which changes aa from Cys to Arg; The genotype of base position described by rs7412 in both parent and subclone is C/C, which is an Arg. Together, these base changes define a ApoE4/E4 genotype in the subclone. Please see chromatogram for confirmation of the base changes in the subclone

General information

Cell line name:BIONi010-C-4
Cell line alternative names:BIONi010-C ApoE E4/E4 #B44 P27
Biosamples ID:SAMEA4452060
hPSCreg name:BIONi010-C-4
Derivation country:Denmark

Related lines

Derived from: BIONi010-C
All subclones of BIONi010-C: BIONi010-C-10, BIONi010-C-11, BIONi010-C-12, BIONi010-C-13, BIONi010-C-15, BIONi010-C-17, BIONi010-C-18, BIONi010-C-19, BIONi010-C-2, BIONi010-C-24, BIONi010-C-25, BIONi010-C-3, BIONi010-C-4, BIONi010-C-5, BIONi010-C-6, BIONi010-C-7, BIONi010-C-8, BIONi010-C-9
From the same donor: BIONi010-A, BIONi010-B
Timepoint: Confluence
Magnification: x10
Timepoint: Confluence
Magnification: x4
This line is part of a set of isogenic APOE lines based on the iPS cell line BIONi010-C. The set comprises the following APOE genotypes: • BIONi010-C-6 (APOE 2/2) • BIONi010-C-2 (APOE 3/3) • BIONi010-C (APOE 3/4) • BIONi010-C-4 (APOE 4/4) • BIONi010-C-3 (APOE KO) A DNA SNP array revealed no larger chromosomal aberrations to be reported. An unsignificant duplication of 1,4Mbp was found on Chr22 in q11.23. This duplication was found in all isogenic lines generated from BIONi010-C as well as BIONi010-C. Other isogenic ApoE cell line cohorts are also available, generated from BIONi37-A, UKBi011-A and STBci006-A.
At European Collection of Authenticated Cell Cultures (ECACC)
In stock
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