BIONi010-C-17 Gene-edited iPSC line
Donor information | |
|---|---|
| Gender: | Male |
| Donor karyotype: | 46, XY |
Disease status | |
Donor | |
| Disease: | normal |
Disease/phenotype related genetic modifications |
|
| Disease: | Alzheimer's disease |
| Type of modification: | Gene knock-out |
| Gene: | TREM2 |
| Chromosome location: | 6p21.1 |
| Delivery method: | CRISPR-associated (CRISPR/Cas) System |
| Description: | KO of exon 1 of TREM2 Transcript variant 1 NM_018965 |
General information | |
| Depositor: | Bioneer |
| Cell line name: | BIONi010-C-17 |
| Cell line alternative names: | BIONi010-C TREM2 KO |
| Biosamples ID: | SAMEA104386270 |
| hPSCreg name: | BIONi010-C-17 |
| Derivation country: | Denmark |
Related lines | |
| Derived from: | BIONi010-C |
| All subclones of BIONi010-C: | BIONi010-C-10, BIONi010-C-11, BIONi010-C-12, BIONi010-C-13, BIONi010-C-15, BIONi010-C-17, BIONi010-C-18, BIONi010-C-19, BIONi010-C-2, BIONi010-C-24, BIONi010-C-25, BIONi010-C-3, BIONi010-C-4, BIONi010-C-5, BIONi010-C-6, BIONi010-C-7, BIONi010-C-8, BIONi010-C-9 |
| From the same donor: | BIONi010-A, BIONi010-B |
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Derivation
Derivation
Primary cell type: - Reprogramming method
Vector type: Non-integrating Vector: Episomal Is the reprogramming vector detectable: Unknown Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Surface coating: Matrigel/Geltrex Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 20% CO2 concentration: 5% Culture medium: Essential 8™ Passage Number banked (pre-EBiSC): 35 No. Vials banked (pre-EBiSC): 30 -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles SSEA-3 Yes SSEA-1 No SSEA-4 Yes SOX2 Yes POU5F1 (OCT-4) Yes Morphology: TREM2 KO cl. 24-54 D1 thaw aft_ekU7aDe.tif - Phase contrast image 1 day after thawing
Differentiation potency
Ectoderm: Ectoderm In vitro directed differentiation
Morphology:
Protocol or reference:
BIONi010-C-17 ecto D5.tif - Flow cytometry of in vitro differentiation to ectodermEndoderm: Endoderm In vitro directed differentiation
Morphology:
Protocol or reference:
BIONi010-C-17 endo D5.tif - Flow cytometry of in vitro differentiation to endodermMesoderm: Mesoderm In vitro directed differentiation
Morphology:
Protocol or reference:
BIONi010-C-17 meso D5.tif - Flow cytometry of in vitro differentiation to mesodermMicrobiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative -
Genotyping
Genotyping
Karyotyping
Passage number: 35 Cell line karyotype: 46, XY Karyotyping method: G-Banding Image / Result -
Genetic modification
Genetic modification
Disease/phenotype related modifications
Disease: Alzheimer's disease Type of modification: Gene knock-out Gene: TREM2 Chromosome location: 6p21.1 Delivery method: CRISPR-associated (CRISPR/Cas) System Description: KO of exon 1 of TREM2 Transcript variant 1 NM_018965
No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23
At European Collection of Authenticated Cell Cultures (ECACC)
Restocking
Cell Line Information Pack
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