BIONi010-C-17
BIONi010-C TREM2 KO
Gene-edited iPSC line
At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.
General#
Cell Line |
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| hPSCreg Name | BIONi010-C-17 |
| Alternative name(s) |
BIONi010-C TREM2 KO
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Notes | No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23 |
Provider |
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| Depositor | Bioneer (BION) |
| Owner | Bioneer (BION) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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| Derivation country | Denmark |
External Databases |
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| hPSCreg | BIONi010-C-17 |
| BioSamples | SAMEA104386270 |
| ECACC | 66540632 |
| Cellosaurus | CVCL_RM88 |
| CLO | CLO_0102729 |
| Wikidata | Q54796761 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research: allowed
Clinical: allowed
Commercial: allowed
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| Subclone of | |
Donor Information#
General Donor Information |
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| Sex | male |
| Ethnicity | Black or African-American |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA3105780 |
hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
BIONi010-C.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| SOX2 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| SSEA-1 |
No |
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Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Morphology pictures
TREM2 KO cl. 24-54 D1 thaw after banking.tif
Phase contrast image 1 day after thawing
Method documentation
06-Sep-2017-Layout.tiff
Flow Cytometry with pluripotency markers
qPCR BIONi010-C-17.tif
qPCR with pluripotency markers
Differentiation Potency
In vitro directed differentiation
Morphology
Protocol or reference
BIONi010-C-17 endo D5.tif
Flow cytometry of in vitro differentiation to endoderm
In vitro directed differentiation
Morphology
Protocol or reference
BIONi010-C-17 meso D5.tif
Flow cytometry of in vitro differentiation to mesoderm
In vitro directed differentiation
Morphology
Protocol or reference
BIONi010-C-17 ecto D5.tif
Flow cytometry of in vitro differentiation to ectoderm
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Sterility |
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| Inoculation for microbiological growth | No Contaminants Detected |
| Mycoplasma | Not Detected |
| Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|
Genetic Modification#
| Disease/phenotype related modifications |
|