BIONi010-C-12

HNF4ApR309C -/- 2-4

Gene-edited iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name BIONi010-C-12
Alternative name(s)
HNF4ApR309C -/- 2-4
Cell line type Human induced pluripotent stem cell (hiPSC)
Notes No larger chromosomal aberrations to be reported. Chr22: 1,4Mbp duplication in q11.23

Provider

Depositor Bioneer (BION)
Owner Bioneer (BION)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country Denmark

External Databases

hPSCreg BIONi010-C-12
BioSamples SAMEA104619383
ECACC 66541076
Cellosaurus CVCL_RM86
CLO CLO_0102726
Wikidata Q54796756

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed
Subclone of

Donor Information#

General Donor Information

Sex male
Ethnicity Black or African-American

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA3105780

hIPSC Derivation#

General

The source cell information can be found in the parental cell line BIONi010-C.

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Method documentation
HNF4A 2-4 flow.tif
Flow cytometry of undifferentiated cells

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Passage number: 12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Genetic Modification#

Disease/phenotype related modifications
MODY
Synonyms
  • Maturity-onset diabetes of the young
Genetic modifications
HNF4A (target)
Isogenic modification
20q13.12
NM_175914.4:c.925C>T
NP_787110.2:p.(p.Arg309Cys)
Homozygous
A Cytocine to Tyrosine mutation was introduced into both alleles of exon 8 in HNF4A resulting in a mutation previously shown to cause MODY1 in patients.
Mutated