BIHi031-B

iPSC line

At European Collection of Authenticated Cell Cultures (ECACC)
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg Name BIHi031-B
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Berlin Institute of Health (BIH)
Owner Berlin Institute of Health (BIH)
Distributors
Berlin Institute of Health (BIH)
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country Germany

External Databases

hPSCreg BIHi031-B
BioSamples SAMEA104493010
ECACC 66540642
Cellosaurus CVCL_VC50
CLO CLO_0101976
Wikidata Q54796677

General Information

* Is the cell line readily obtainable for third parties?
Yes
Clinical: not allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 35-39

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
focal segmental glomerulosclerosis
Synonyms
  • glomerulosclerosis, focal
  • glomerulosclerosis
  • glomerulosclerosis, focal segmental
  • glomerulonephritis, focal sclerosing
  • focal glomerulosclerosis
  • FGS
  • focal glomerular sclerosis
  • FSGS
  • FSGS - focal segmental glomerulosclerosis
  • FGS (focal glomerular sclerosis)
  • focal segmental glomerulosclerosis
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493011

hIPSC Derivation#

General

Source cell type
peripheral blood mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 35-39
Collected in 2016

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Selection criteria for clones typical hPSC morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
Pluripotency Score Novelty Score
33.94 1.49
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: KaryoLite BoBs

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
SNP typing array
no mutations detected by virtual karyotyping