BIHi006-D
Donor information | |
|---|---|
| Gender: | Male |
| Age: | 35-39 |
Disease status | |
| Disease: | focal segmental glomerulosclerosis |
General information | |
| Depositor: | Berlin Institute of Health |
| Cell line name: | BIHi006-D |
| Cell line alternative names: | - |
| Biosamples ID: | SAMEA104493310 |
| hPSCreg name: | BIHi006-D |
| Derivation country: | Germany |
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Derivation
Derivation
Primary cell type: Peripheral blood mononuclear cell Tissue collection year: 2016 Reprogramming method
Vector type: Non-integrating Vector: Sendai virus Gene list: - KLF4
- MYC
- POU5F1
- SOX2
Is the reprogramming vector detectable: No Merthods used: pcr Derivation
Xeno free conditions: no Derived under gmp: no Available as clinical grade: no -
Culture conditions
Culture Conditions
Surface coating: Matrigel/Geltrex Passage method: Enzyme-free cell dissociation / EDTA O2 concentration: 5% CO2 concentration: 5% Culture medium: Essential 8™ Passage Number banked (pre-EBiSC): 16 No. Vials banked (pre-EBiSC): 35 -
Characterization
Characterization
Analysis of Undifferentiated Cells
Marker expression: Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles SSEA-4 Yes TRA 1-60 Yes NANOG Yes POU5F1 (OCT-4) Yes PluriTest: Pluripotency score: 28.27
Novelty score: 1.42The PluriTest is a pluripotency assessment based on DNA microarray data, which considers the genome-wide expression profile of the sample (www.nature.com/nmeth/journal/v8/n4/full/nmeth.1580.html).
PluriTest scores should be assessed in the context of the empirical distribution of pluripotent and somatic samples used to construct and validate PluriTest (e.g. Exemplary use of PluriTest). The training set of iPSC clustered with pluripotency score > 25 and novelty score < 1.67 with 98% sensitivity and 100% specificity. Cell lines with pluritest scores > 10 also cluster with the PluriTest training data set with high sensitivy and specificity.
Differentiation potency
Ectoderm: Ectoderm In vitro directed differentiation
Endoderm: Endoderm In vitro directed differentiation
Mesoderm: Mesoderm In vitro directed differentiation
Microbiology / Virus Screening
HIV 1: Negative HIV 2: Negative Hepatitis B: Negative Hepatitis C: Negative Mycoplasma: Negative -
Genotyping
Genotyping
Karyotyping
Cell line karyotype: 46,XY Karyotyping method: KaryoLite BoBs Genotyping
Genome-wide analysis: SNP typing array
At European Collection of Authenticated Cell Cultures (ECACC)
Restocking
Cell Line Information Pack
A Cell Line Information Pack (CLIP) is created to communicate cell line specific information relating to restrictions on use to a user accessing cell lines from EBiSC. A CLIP contains information about a cell line including any specific third party obligations (TPOs) relating to, for example, intellectual property (IP) or the donor consent which affect the use of the cell line.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.